Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

Search Within Results

106 – 120 of 3,503 results

106

Thermo Scientific Chemicals HEPES-buffered saline (5X, pH 7.4), low endotoxin

Pricing & Availability
Specifications

Name Note	low endotoxin
Storage Note 1	2° to 8°C
Color	Colorless
Physical Form	Liquid
Chemical Name or Material	HEPES-buffered saline (5X, pH 7.4)
TSCA	Yes
Recommended Storage	Keep cold

107

Promotions Available

Thermo Scientific Chemicals MOPS, 99%, for biochemistry

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-(N-Morpholino)propanesulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

Pricing & Availability
Specifications

PubChem CID	70807
CAS	1132-61-2
Molecular Weight (g/mol)	209.26
ChEBI	CHEBI:44115
MDL Number	MFCD00006183
SMILES	[O-]S(=O)(=O)CCC[NH+]1CCOCC1
Synonym	3-(N-Morpholino)propanesulfonic acid
InChI Key	DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula	C7H15NO4S

108

Promotions Available

MOPS sodium salt, 98%

CAS: 71119-22-7 Molecular Formula: C7H14NNaO4S Molecular Weight (g/mol): 231.242 MDL Number: MFCD00064350 InChI Key: MWEMXEWFLIDTSJ-UHFFFAOYSA-M Synonym: 3-(4-Morpholino)propanesulfonic acid sodium salt PubChem CID: 3859613 IUPAC Name: sodium;3-morpholin-4-ylpropane-1-sulfonate SMILES: C1COCCN1CCCS(=O)(=O)[O-].[Na+]

Pricing & Availability
Specifications

PubChem CID	3859613
CAS	71119-22-7
Molecular Weight (g/mol)	231.242
MDL Number	MFCD00064350
SMILES	C1COCCN1CCCS(=O)(=O)[O-].[Na+]
Synonym	3-(4-Morpholino)propanesulfonic acid sodium salt
IUPAC Name	sodium;3-morpholin-4-ylpropane-1-sulfonate
InChI Key	MWEMXEWFLIDTSJ-UHFFFAOYSA-M
Molecular Formula	C7H14NNaO4S

109

Promotions Available

Tris(hydroxymethyl)aminomethane, 99.8%, ACS reagent

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-(hydroxymethyl)-1, 3-propanediol,TRIS,Tris buffer,Tromethamine,Tromethane PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
Synonym	2-Amino-2-(hydroxymethyl)-1, 3-propanediol,TRIS,Tris buffer,Tromethamine,Tromethane
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

110

Promotions Available

Bicine, 99+%, for analysis

CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: N, N-Bis(2-hydroxyethyl)glycine,Diethylolglycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: [Na+].OCCN(CCO)CC([O-])=O

Pricing & Availability
Specifications

PubChem CID	8761
CAS	150-25-4
Molecular Weight (g/mol)	185.16
ChEBI	CHEBI:40957
MDL Number	MFCD00004295
SMILES	[Na+].OCCN(CCO)CC([O-])=O
Synonym	N, N-Bis(2-hydroxyethyl)glycine,Diethylolglycine
IUPAC Name	2-[bis(2-hydroxyethyl)amino]acetic acid
InChI Key	MFBDBXAVPLFMNJ-UHFFFAOYSA-M
Molecular Formula	C6H12NNaO4

111

Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 8.5

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

112

TAE Buffer, 10X Solution, Molecular Biology Grade, Ultrapure

Pricing & Availability
Specifications

Physical Form	Solution
Chemical Name or Material	TAE Buffer
Grade	Molecular Biology, Ultrapure
TSCA	Yes
Recommended Storage	Ambient temperatures
Concentration	10X

113

Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 9.5

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

114

MP Biomedicals™ Phosphate Buffered Saline Tablets (PBS)

Phosphate Buffered Saline Tablets (PBS) are a balanced salt mixture for easy PBS buffer preparation for a variety of applications, including reagent preparation, diluting cells for flow cytometry, and cell culture reagent.

Pricing & Availability
Specifications

Includes	No Calcium, No Magnesium
Molecular Weight (g/mol)	74.55
MDL Number	MFCD00011360
Physical Form	Tablet
Chemical Name or Material	Phosphate Buffered Saline (PBS)
SMILES	[Cl-].[K+]
IUPAC Name	potassium chloride
InChI Key	WCUXLLCKKVVCTQ-UHFFFAOYSA-M
Molecular Formula	ClK

115

Thermo Scientific Chemicals MES, 0.5M buffer soln., pH 6.0

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1

Pricing & Availability
Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.25
MDL Number	MFCD00149409
SMILES	O.OS(=O)(=O)CCN1CCOCC1
IUPAC Name	2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

116

Thermo Scientific™ Pierce™ Endotoxin-Free Tris Buffer, pH 7.0 (50 mM)

Pierce Endotoxin-Free Tris Buffer may be used to dilute samples for any of the endotoxin detection assays and for adjusting the pH of the LAL endotoxin test samples. For endotoxin testing, the samples and sample dilutions be between pH 6-8. Contains <0.005 EU/mL endotoxin.

Pricing & Availability

117

PBS, 10X Solution, Molecular Biology Grade

For biological research. Affymetrix/USB™ PBS, 10X Solution, Molecular Biology Grade is 0.2μm filtered and dispensed into durable, square nalgene bottles and upright carton.

Pricing & Availability
Specifications

Formulation	80.6 mM sodium phosphate, 19.4 mM potassium phosphate, 27 mM KCl and 1.37 M NaCl in high purity dH₂O
Physical Form	Solution
pH	7.4
Recommended Storage	Ambient temperatures

118

Promotions Available

PIPES, 98%

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N Synonym: pipes,1,4-piperazinediethanesulfonic acid,piperazine-n,n'-bis 2-ethanesulfonic acid,2,2'-piperazine-1,4-diyl diethanesulfonic acid,1,4-piperazinebis ethanesulfonic acid,unii-g502h79v6l,2-4-2-sulfoethyl piperazin-1-yl ethanesulfonic acid,piperazine-1,4-bis 2-ethanesulfonic acid,1,4-piperazine-diethanesulfonic acid,2,2'-piperazine-1,4-diyl bis ethanesulphonic acid PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

Pricing & Availability
Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36
ChEBI	CHEBI:44933
MDL Number	MFCD00006159
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
Synonym	pipes,1,4-piperazinediethanesulfonic acid,piperazine-n,n'-bis 2-ethanesulfonic acid,2,2'-piperazine-1,4-diyl diethanesulfonic acid,1,4-piperazinebis ethanesulfonic acid,unii-g502h79v6l,2-4-2-sulfoethyl piperazin-1-yl ethanesulfonic acid,piperazine-1,4-bis 2-ethanesulfonic acid,1,4-piperazine-diethanesulfonic acid,2,2'-piperazine-1,4-diyl bis ethanesulphonic acid
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

119

Phosphate Buffered Saline, 1X - Non-sterile, Ricca Chemical

Phosphate Buffered Saline, 1X is non-sterile.

Pricing & Availability

120

TE Buffer, 1X Solution, Molecular Biology Grade, Ultrapure

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

Pricing & Availability
Specifications

PubChem CID	93573
CAS	1185-53-1
Molecular Weight (g/mol)	157.594
SMILES	C(C(CO)(CO)N)O.Cl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

Life Science Buffers

Filtered Search Results

Narrow Results